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This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.
RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.
* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center
* Includes classic and contemporary techniques
* Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
- Published on: 2010-07-22
- Released on: 2010-07-22
- Format: Kindle eBook
Review
Praise for the third edition
"This third edition of RNA Methodologies is an updated version of a laboratory guide edited for the first time in 1993; it represents an exhaustive collection of tried, tested, and optimized protocols for isolation and chracterization of RNA. The third edition of RNA Methodologies is a well-documented guide for any researcher who has to manage RNA studies. Each chapter of this book provides a rationale to help in the decision-making process...The inclusion of troubleshooting hints and annotations based on personal experience are provided to improve the method(s). This laboratory guide offers protocols on traditional and new technologies, with a focus on how to use the expedient technique for achieving an experimental goal in the study of gene expression."
--Dr. Patrick Pagesy at INSERM for HEMOGLOBIN
From the Back Cover
The isolation of biologically competent and chemically stable RNA is central to molecular biology. There remains an academic imperative to unify the many complex facets of the characterizations of cellular biochemistry with particular regards to the use of RNA as a parameter of gene expression. This laboratory guide is a growing collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. The focus herein is how these methodologies can be used in the study of transcriptional- and post-transcriptional regulation of gene expression.
KEY FEATURES:
*New sections describing RNA isolation, subtraction-suppression PCR, high-throughput methods, bioinformatics, and RNAi
*Explains proper handling, storage, and manipulation of RNA
*An expanded section on RT-PCR, RACE, and related techniques
*Trouble-shooting options are incorporated throughout the text
Most helpful customer reviews
5 of 5 people found the following review helpful.
great methods book
By A Customer
This book is exactly what it claims to be--a laboratory guide. It provides methods that are clear, but also explains the reasons for the methods. For example: when is an RNA probe better than a DNA probe and why; when nonisotopic labels can be used effectively. Although there is not a chapter on in situ hybridization, I found the section on probes very helpful. There is also a handy glossary at the end and a primer on RNA at the beginning of the book. Dr Farrell realizes the reader maybe new to the field and so doesn't leave out the basics. However, I believe this book will be helpful to all but the most knowledgable molecular biolgists.
1 of 3 people found the following review helpful.
Not advanced and back-dated
By citu
I am a RNA-chemist and I bought this book from amazon.com to get help for my day-to-day research. The moment I had my first glance at this book I was frustrated. This book has described fairly old methods but no contemporary techniques. This book is not even descrided the techniques in an advanced manner which is expected for a book based on so specific field. I would not recommend this book to anyone who has atleast the basic working knowledge of molecular biology.
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